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產品介紹
品名	monoclonal anti-Glutamate antibody
貨號	mAB 2D7
規格	200 μl
宿主動物	Mouse monoclonal, IgG1
抗體形式	Conc. supernat. lyophil.
克隆性	monoclonal
產品形式	Lyophilized (no preservatives)
產品描述	The antibodies have been purified from ascitic fluid by ammoniumsulfate precipitation and subsequently by FPLC on Protein-A-Superose. BSA has been added to the eluate, and the material has been dialyzed against water and lyophilized.
產品重構	- add the lyophilized material (approx. 1 mg) to 100 μl PBS or another buffered saline (add in order indicated)  - allow some time for the material to dissolve; mix by swirling gently  - remove particulate matter by centrifugation in a microfuge - add 100 μl glycerol (final glycerol conc. 50% v/v) and mix well (vortex)
產品稀釋	Appropriate final dilutions should be determined experimentally. For LM, we strongly suggest to use postembedding staining and to combine the anti-Glu antibody with a silver-enhanced PAP procedure as described in detail by Liu et al.(1989). For EM, we recommend a postembedding immunogold procedure (see: Liu et al., 1989).
儲存	Store stock-solution in freezer (-20°C, not -70°C). Mix stock solution well before making aliquots. 10x diluted solutions may be kept in the refrigerator for some time (several weeks) but should be made 10 mM with sodium azide. Volumes of working dilutions (high dilutions) should be calculated such the they are used up the same day. Avoid polystyrene tubes.
參考文獻	Liu CJ, Grandes P, Matute C, Cuenod M, Streit P: Glutamate-like immunoreactivity revealed in rat olfactory bulb, hippocampus and cerebellum by monoclonal antibody and sensitive staining method. Histochemistry 1989;90(6):427-45