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Y-27632 is a widely used, selective inhibitor of the Rho-associated protein kinase p160ROCK with a Ki value at ~ 0.14 μM. In many studies, it was shown and used to enhance the survival and cloning efficiency of many primary cell types after single cell dissociation without affecting their self-renewal properties, including hESC/iPSCs, neural stem cells, intestinal stem cells, and mammary epithelial cells.

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How to Use:

• In vitro: Y-27632 is typically used at 10 μM concentration in cell culture.
• In vivo: In several hypertension rat models, Y-27632 was orally dosed at 30 mg/kg to significantly decrease blood pressure.?

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Reference:

1. 1. Uehata M, et al. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension.(1997) Nature. 389(6654):990-4.
2. 2. Ishizaki T. et al. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. (2000) Mol Pharmacol 57, 976-983.
3. 3. Koyanagi et al. Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. (2008) J. Neurosci Res 86: 270-280.
4. 4. Watanabe et al.? A ROCK inhibitor permits survival of dissociated human embryonic stem cells. (2007) Nat Biotech 25: 681-686.

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